PCR mycoplasma detection kit-盟基生物科技股份有限公司

Mycoplasma (黴漿菌相關產品)

PCR mycoplasma detection kit

Mycoplasma (黴漿菌相關產品)

PCR mycoplasma detection kit

超省時又省力的黴漿菌檢測套組，只需要取細胞上清液進行 PCR 反應

一、產品介紹

黴漿菌是一種微生物，為沒有細胞壁的原核生物。大小 0.2-0.3 um，而且細胞型態不固定，因此有一定機率通過0.22 um 過濾膜，造成細胞汙染。由於黴漿菌沒有細胞壁，因此一般的抗生素無法清除黴漿菌，需要使用特別的抗生素 (請見產品 “Mycoplasma removal agent”)。黴漿菌為實驗室細胞培養常見的汙染源，與一般的細菌真菌汙染不同，黴漿菌汙染不會造成培養液混濁，也不會造成細胞死亡，因此不容易以顯微鏡和肉眼觀察。然而，黴漿菌的污染卻會造成細胞的改變，影響細胞所有的特性和行為，造成實驗結果上的誤差和錯誤。常見的現象有細胞生長速度緩慢、細胞轉染效率降低、細胞生產病毒效率降低以及培養液快速地變色。以高倍顯微鏡觀察下，可以看到奇怪的小黑點。
黴漿菌檢測的方法有很多，包括螢光檢測、菌盤培養法和抗體偵測，這些方法的缺點在於需要較多時間操作實驗，或需要特殊的儀器設備。Abm 研發的黴漿菌檢測套組使用上非常簡單，不須純化 DNA，直接以細胞上清液做傳統 PCR 反應，以電泳分析結果 (370-550 bp)，可於二小時內完成反應。

NIH3T3 細胞有無汙染的構造，左圖為無汙染的細胞，表面光滑和平坦。右圖則為黴漿菌汙染的細胞，表面非常粗糙和有許多凸起。

對於這些令人頭痛的mycoplasma與病毒，盟基生物科技提供T-pro MycoClean spray，具有極佳的潔淨能力，例如：staphyhococcus aureus、streptococcus faecalis、Escherichia coli、Proteus mirabilis、Pseudomonas aeruginosa、Candida albicans、HBV、Papova-、Adeno-、Polio- and Vaccinia Viridae等。您只需要在欲清潔之表面噴上T-Pro MycoClean並於兩分鐘後擦拭或讓它在十分鐘內自然自然揮發掉即可。只需要一點點，就有不可思議的驚人效果。

二、產品特色

1. 操作超簡單：
直接取細胞培養的上清液做反應，以電泳分析，不需要純化 DNA (也可以使用純化後的 DNA 反應)。為傳統 PCR 操作，以電泳觀察，不須額外訓練。

Abm 黴漿菌檢測套組的流程示意圖，僅需取細胞培養的上清液做 PCR 反應即可。

2. 常規使用的抗生素 (penicillin, streptomycin, and gentamicin) 不影響實驗結果

3. 偵測範圍超廣：
可偵測200種以上黴漿菌

可被檢測到的黴漿菌菌種
verecundum	fermentans M64	zalophi strain CSL 4296	hyopneumoniae J
verecundum strain ATCC 27862	fermentans JER	zalophi strain CSL 5195	hyopneumoniae 168
gallisepticum strain XZA	fermentans strain ATCC 49892	zalophi strain CSL 4296	hyopneumoniae strain ATCC 27714
gallisepticum strain JXB	vulturii strain Gb‐V33	gateae strain KDC	hyopneumoniae 7448
gallisepticum strain YMC	vulturii strain Gb‐V33	gateae strain V‐1	hyopneumoniae 232
gallisepticum strain ML	edwardii strain ATCC 23462	gateae strain ATCC 23392	zalophidermidis strain CSL 4779
gallisepticum strain WSA	edwardii strain 04‐3436	canadense strain 466	pulmonis strain Ash (PG34)
gallisepticum strain JXA	edwardii strain 04‐3440	canadense strain 275C	pulmonis (strain UAB CTIP)
gallisepticum strain WSB	flocculare	canadense strain ATCC 29418	alligatoris strain ATCC 700619
gallisepticum strain XZC	flocculare strain Ms42	canadense strain 466	crocodyli
gallisepticum strain F	conjunctivae HRC/581T	corogypsi strain BV1‐5	crocodyli MP145
gallisepticum strain MG‐HS	conjunctivae strain Goat 655	corogypsi strain BV1‐1	crocodyli strain ATCC 51981
gallisepticum strain YMA	conjunctivae strain Goat 655	equirhinis strain M432‐72	subdolum strain ATCC 29870
gallisepticum strain XA	conjunctivae strain HRC 581	equirhinis strain ATCC 29420	phocicerebrale strain CSL 5195S2
gypis	phocicerebrale ATCC 51405	canis strain ATCC 19525	phocicerebrale strain ATCC 51405
gypis strain ATCC 51370	phocicerebrale strain 1049	canis strain C3b	phocicerebrale strain Cheryl
indiense	hyorhinis GDL‐1	canis strain Skotti A	bovis
gallinaceum	hyorhinis clone V116‐1	hyopharyngis strain H36B‐F	bovis strain HEK‐FDA
gallinaceum strain SA	hyorhinis strain GDL	hyopharyngis strain 538 Nasal	bovis HB0801
gallinaceum strain ATCC 33550	hyorhinis ATCC:29052	auris	bovis PG45 clone MU clone A2
gallinaceum strain 887	hyorhinis isolate LuSIV	cloacale	bovis strain Madison
gallinaceum strain 111R	hyorhinis strain ATCC 29052	mustelae	bovis Hubei‐1
gallinaceum strain 33F	hyorhinis MCLD	mustelae strain MX9	bovis strain 70‐213 (Del Giudice)
arginini	sp. Phocoena C‐264‐GEN	sp. 1220	bovis strain ATCC 25025
arginini strain EF‐Hungary	sp. Phocoena C‐269	sp. 31848	gallinarum strain ATCC 15319
arginini strain ATCC 23243	agalactiae	sp. 31948	gallinarum strain B2 (Dinter)
arginini clone V111‐1	agalactiae PG2	salivarium	gallinarum ATCC 15319
sphenisci strain UCMJ	agalactiae 5632	salivarium strain MP1166	phocirhinis strain CSL7520‐lung
sp. Mirounga ES2783_AG_GEN	neurolyticum strain ATCC 19988	leonicaptivi strain ATCC 49890	phocirhinis strain CSL 7475‐4
sp. Mirounga ES2774‐NAS	neurolyticum strain 10RN‐F‐3	anatis	phocirhinis strain ATCC 49639
sp. Mirounga ES2806‐NAS	neurolyticum strain T15	anatis strain K6193C	collis strain ATCC 35278
sp. Mirounga ES2774‐NASSP4	neurolyticum strain 36CFA2	anatis strain K6193A	californicum strain ST6
sp. Mirounga ES2714_AG_GEN	agassizii strain PS6	felis strain ATCC 23391	californicum strain ATCC 33461
sp. Mirounga ES2805‐ORL	agassizii strain Karina 09 Fr. Tortoise	anseris	spumans
pullorum	falconis ATCC 51372	hyopharyngis	buteonis strain Bb/T2g
pullorum strain ATCC 33553	lagogenitalium strain ATCC 700289	bovirhinis	oxoniensis
gallopavonis strain WR1	ovipneumoniae strain ATCC 29419	simbae ATCC 49888	leopharyngis
gallopavonis strain ATCC 1197	columbinasale strain ATCC 33549	synoviae ATCC 25204	primatum strain HRC292
sp. CSL7489‐oral	felifaucium strain PU	synoviae 53	primatum strain I30
sp. CSL7529‐conjunctiva	spermatophilum	orale	primatum strain ATCC 25948
sp. CSL7491‐lung	spermatophilum strain AH159	orale strain MT‐4	molare strain ATCC 27746
sp. CSL7518‐lung	spermatophilum strain 88(Jacobs)	hyosynoviae strain ATCC 25591	lipofaciens strain ATCC 35015
dispar strain ATCC 27140	bovigenitalium strain MH 1113	bovoculi strain ATCC 29104	cricetuli
arthritidis strain 91021	bovigenitalium strain PG‐11	maculosum strain ATCC 19327	glycophilum
arthritidis strain ATCC 19611	bovigenitalium strain ATCC 14173	maculosum strain Skotti B	citelli strain RG‐2C
arthritidis 158L3‐1	bovigenitalium strain ATCC 19852	elephantis strain ATCC 51980	citelli strain RG1D‐L
meleagridis	equigenitalium strain ATCC 29869	columbinum	citelli strain RG1D‐S
meleagridis strain ATCC 27764	iguanae strain 2327	columbinum strain FG295	citelli strain RG2C
iners strain PG30	testudineum strain ATCC 700618	caviae strain G122	citelli strain RG1D‐L
iners strain 89R‐D‐3	testudineum strain BH29	caviae strain ATCC 27108	columborale strain ATCC 29258
iners strain 1D‐E‐3	moatsii strain MK405	hominis ATCC 23114	columborale strain 18303
iners strain ATCC 15969	opalescens	hominis strain ATCC 27545	sturni ATCC 51945

4. 偵測超靈敏：
只有10個黴漿菌/sample 也可以檢測到

5. 省時：
反應小於2小時

6. 附上正控制組：
為包含黴漿菌 gDNA 的載體，確保實驗步驟正確以及無 PCR inhibitor 干擾

三、產品資訊

貨號	品名	包裝
G238	PCR Mycoplasma Detection kit	100 reactions

四、相關產品資訊

貨號	品名	包裝
JT90-R002	T-pro MycoClean Spray	500 ml
093050044	Mycoplasma Removal Agent (MRA)	5 ml

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