Cell Suspension Dissociator 活細胞組織酶解儀-盟基生物科技股份有限公司

客製化抗體-盟基生物科技股份有限公司

活細胞組織酶解儀

Cell Suspension Dissociator 活細胞組織酶解儀

Cell Suspension Dissociator 活細胞組織酶解儀

活細胞組織酶解儀

Cell Suspension Dissociator 活細胞組織酶解儀

酵素酶解試劑套組搭配儀器程式化自動處理組織,以獲得高品質、高存活率之單細胞。

一、產品介紹
RWD活細胞組織酶解儀,通過自行研發的組織處理管+酶解試劑套組,搭配內建對應組織優化的處理程式,可將組織在15-30mins內製備成高存活率、均一性好的單細胞懸浮液或組織均質勻漿。擁有獨立運行的多通道, 搭配加熱套實現全自動化處理,提高組織處理效率,大幅提升實驗的可重複性。




二、產品優勢
1. 一機兩用,可製備單細胞懸液或組織均質勻漿
2. 多通道獨立操作,可同步處理不同組織,節省實驗時間
3. 內建標準化處理程式,直接選用操作簡單,支援另存500組自訂程式
4. 電機控制轉速平穩可靠,滿足0~4000轉/min的不同應用需求
5. 配置加熱套,恆溫37℃、酵素作用更完全


三、產品應用
1. Cell therapy
2. Flow cytometry, cell sorting
3. Primary cell culture
4. Single cell sequencing



四、產品資訊
1. 操作流程



2. 實驗驗證
✦ 細胞活性佳、初代培養沒問題
使用RWD組織酶解儀處理小鼠肝臟組織後,立即以顯微鏡觀察僅有少數細胞團塊及碎片(左上圖: 明視野觀察、左下圖: PAS染色結果)。
培養3天後,細胞貼壁生長良好(右上圖: 明視野觀察、右下圖: PAS染色結果)。
 

 

使用RWD組織酶解儀處理小鼠肺癌組織(LLC),利用Flow cytometry分析細胞存活率及產量,結果顯著優於傳統手工處理組織方式。
 
 
使用RWD組織酶解儀處理小鼠多種組織,結果顯示組織處理後細胞存活率高(左圖)以及每100mg組織回收之細胞數量(右圖)
 

 
使用RWD組織酶解儀處理小鼠小腸組織,先利用RWD奈米磁珠系統分選帶有特定抗原(CD45、CD19)之細胞,再以Flow cytometry 分析細胞表面抗原(CD45、CD19)。結果顯示無論是帶有CD45(左圖)或CD19(右圖)抗原的細胞群,分選後的細胞純度極高。
 
 
 
Channels 2 4 8
Temperature 37 °C
Speed 0~4000rpm
Sample Volume 20~4000mg
Buffer Volume 0.3~10mL
Screen 7 inches, touch screen
User Programs default programs+500 user programs default programs+custom programs+500 storable programs
Weight 9kg 10.3 kg 18 kg
Dimensions (W*D*H) 215*380*190 mm 215*380*190 mm 415*381*256 mm
Tissue Processing Tube組織處理管

一、產品介紹

1. 經過Ethylene oxide(EO)滅菌處理與整體封裝
2. 獨立包裝,安全無菌

 


二、產品資訊
型號 樣品處理量 緩衝液體積 尺寸 包裝
SCT-25, SCT-100 20-4000 mg 0.3-10ml (L*H)35*89 mm

25 pcs/box

100 pcs/box



酶解試劑套組



一、產品介紹
1. 經過優化的酶解配方,確保得到高存活率的活細胞懸浮液
2. 不損傷細胞表面表位,滿足下游實驗順利進行


二、產品資訊
Cat No. Product name Type of tissues Type of cells Specifications
DHTE-5001 High Activity Tumor Tissue Enzymatic Digestion Kit (Mouse) Mouse tumor Tumor cells and immune cells 50T
DHNBE-5002 High Activity Neonatal Brain Enzymatic Digestion Kit (Mouse and Rat) P < 7 brain tissues of mouse and rat Neural stem cells, neurons, astrocytes,oligodendrocytes, glial cells, microglia, endothelial cells 50T
DHABE-5003 High Activity Adult Brain Enzymatic Digestion Kit (Mouse and Rat) P≥7 brain tissues of mouse and rat Neurons, astrocytes, oligodendrocytes, glial cells, microglia,endothelial cells 50T
DHGT-5004 High Activity General Tissue Enzymatic Digestion Kit Heart,liver,spleen,lung, kidney,etc. Non-parenchymal cells and immune cells 50T
DHTEH-2505 High Activity Tumor Tissue Enzymatic Digestion Kit (Human) Human tumor Tumor cells and immune cells 25T
DHDR-5006 High Efficiency Debris Removal Kit Brain,heart,liver, etc. / 50T
DHIE-5007 High Activity Intestine Tissue Enzymatic Digestion Kit (Mouse) Mouse intestinallamina propria tissue Mouse lamina propria immune cells 50T
DHBTE-2508 High Activity Brain Tumor Enzymatic Digestion Kit Human and mouse brain tumor Tumor cells and immune cells 50T
DHWSE-2509 High Activity Whole Skin Enzymatic Digestion Kit (Mouse) Mouse skin Keratinocytes, fibroblasts and Langerhans cells 25T
DHAE-5010 High Activity Adipose Tissue Enzymatic Digestion Kit (Mouse and Rat) Mouse and rat adipose Stromal Vascular Fraction cells 25T
DHME-5012 High Activity Muscle Tissue Enzymatic Digestion Kit (Mouse and Rat) Mouse and rat muscle Myoblasts, muscle satellite cells 50T
DHNE-2511 High Efficiency Nuclei Extraction Kit Mammalian tissue / 25T
DHHE-2515 High Activity Hepatocyte Extraction Kit (Mouse and Rat) Mouse and rat liver Hepatocytes,hepatic stellate cells,etc. 15T
DHUTE-2516 High Activity Umbilical Cord Enzymatic Digestion Kit (Human) Human umbilical cord Mesenchymal stem cells,etc. 25T